Roles of auxin on tissue reunion in Arabidopsis wounded stems. (A and B) Control wild type without decapitation. (C) pin1-1. (D and E) Decapitated wild type. Lanolin paste containing distilled water (D.W.) (A and D), 10−3 M TIBA (B), or 10−3 M IAA (E) was applied. Arrowheads indicate position of cut. pi, pith; co, cortex; vb, vascular bundle. Images were taken 7 d after stem cutting. (Scale bars in A–E, 100 μm.) (F) Quantification of endogenous IAA in upper (U; red bar), lower (L; blue bar), or noncut region (N; gray bar). Mean ± SE is shown (n = 3).