Fig. 4.

Accumulation of threonine and AICAR and depletion of acetyl-CoA and mTHF in ES cells treated with TDH inhibitors. Feederless ES cells were treated with 10 μM of the Qc1 TDH inhibitor for 0, 1, 2, 3, and 4 h before extraction of metabolites in 50% aqueous methanol and subsequent LC-MS/MS analysis. Metabolites increasing in abundance as a function of exposure to the Qc1 inhibitor of TDH are shown in red. Metabolites decreasing in abundance are shown in green.