Table 1.

Effect of squalamine on dengue virus infection of human endothelial cells

Squalamine concentration (μg/mL)	Percent cells infected (percent inhibition)
0	38 ± 1 (0)
10	36 ± 4 (5)
20	26 ± 1 (32)
40	15 ± 1 (61)
60	9 ± 1 (76)
100	0 (100)

Monolayer cultures of HMEC-1 cells grown on glass coverslips were pretreated with squalamine for 2 h at the indicated concentrations. Fresh medium was reintroduced, and virus was added to the culture. After 48 h, cells were fixed and immunostained for viral protein E as described in Materials and Methods. For each concentration, the percentage of cells expressing viral E protein was scored; 10 randomly chosen fields were counted per concentration. SD of the mean is noted.