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. 2011 Sep 7;108(38):15960–15965. doi: 10.1073/pnas.1109438108

Fig. 3.

Fig. 3.

Mutagenesis of wild-type CD4. (A) Dot plots showing binding of HLA-DR1 tetramers to yeast cells displaying wild-type CD4, affinity-matured CD4 A1, or CD4 bearing the mutations Q40Y/T45W (CD4-DM), T45W/P48L, or Q40Y/T45W/P48L. Yeast cells were labeled with SAPE-conjugated HLA-DR1 tetramers (1 μM) and anti–c-myc mAb 9E10, followed by AF488-conjugated goat anti-mouse secondary antibody. The boxed populations show HLA-DR1 tetramer binding. (B) Dot plots showing binding of HLA-DR1 monomers to yeast cells displaying wild-type CD4, CD4 A1, or CD4-DM (Q40Y/T45W) with the mutations K35P, F43L, K46R, G47S, S60R, D63R, or S60R/D63R (CD4-TM). Cells were labeled with SAPE-conjugated HLA-DR1 monomers (4 μM) and anti–c-myc mAb 9E10.