Fig. 5.

Results of IVIS analysis of mice inoculated sc (A and B) or ic (D and E) with equal particles of fLuc-expressing chimeric replicons packaged in WT NA-EEEV (A and D) or 71-77 mutant (B and E) structural proteins. Quantitation of fLuc signal in homogenized brain (C) or DLN (F) is shown. Sensitivity of each image (footpad/DLN or brain) was set equally such that pseudocolor images of light emitted could be directly compared. Error bars are SDs, and some are too small to be seen. *P = 0.01; **P = 0.01.