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. 2011 Sep 8;108(38):15798-15803. doi: 10.1073/pnas.1112185108

Fig. 3.

Fig. 3.

The hybrid P/E tRNA (A). Displacement of the ASL of the P/E tRNA (forest) in the hybrid state and P/P tRNA (light blue) in the classical state. The mRNA is colored in magenta in the hybrid state and in light blue in the classical state. Structural alignment is based on the 23S rRNA of the 50S subunit. (B) The conformation of the codon and ASL of the P/E tRNA (P/E tRNA ASL in green and AUG codon in magenta) and the P/P tRNA (Both P/P tRNA ASL and AUG codon are in light blue) in the P site of the 30S subunit. (C) The CCA-end of the tRNA and its interactions with 23S rRNA in the E site of the 50S subunit, showing base stacking of the A76 with residue G2421 and A2422 in the 23S rRNA for both the classical state (light blue) and the hybrid state (CCA-end of the P/E tRNA in green and the 23S rRNA in blue). (D) Superposition of the ASL of the P/P and P/E tRNA shows that (i) the two structures begin to diverge from the base pair G30:C40; and (ii) a bend starts from the base pair U27:A43 in the tRNA body toward the 50S E site. These changes allow the tRNA acceptor arm to swing toward the E site.