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. 2011 Aug 2;12(8):4861–4871. doi: 10.3390/ijms12084861

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© 2011 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Figure 4. — Silibinin triggers cell cycle arrest. (A) The DNA content of cells treated with Silibinin for the indicated times was analyzed by flow cytometry. Cell-cycle distributions after treatment with 100, 200 μM Silibinin for 24 h. DMSO as the negative control. Cell-cycle distributions were assessed by PI staining. The DNA content of 15,000 events was analyzed by flow cytometry; (B) The number of G1, S and G2 phase cells from Figure 4A.

Figure 4. — Silibinin triggers cell cycle arrest. (A) The DNA content of cells treated with Silibinin for the indicated times was analyzed by flow cytometry. Cell-cycle distributions after treatment with 100, 200 μM Silibinin for 24 h. DMSO as the negative control. Cell-cycle distributions were assessed by PI staining. The DNA content of 15,000 events was analyzed by flow cytometry; (B) The number of G1, S and G2 phase cells from Figure 4A.