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. 2011 Aug 5;12(8):4975–4990. doi: 10.3390/ijms12084975

Figure 2.

Figure 2.

Western Blot analysis of CMG2-Fc protein from intact N. benthamiana plants reduced with 90 mM dithiothreitol (DTT). Samples shown below were obtained from plants harvested at either 3.5 or 14 days post-infiltration (DPI). Lanes 1 and 2 show CMG2-Fc expression on intact plants: CMG2-Fc co-expressed with gene silencing suppressor p1 at 3.5 DPI (lane 1); CMG2-Fc without gene silencing suppressor co-expression at 3.5 DPI (lane 2). Lanes 3 and 4 show CMG2-Fc expression on detached leaves: CMG2-Fc co-expressed with gene silencing suppressor p19 at 14 DPI (lane 3); CMG2-Fc without gene silencing suppressor co-expression at 14 DPI (lane 4). Lane M: molecular weight marker; lane WTi: control infiltration of an intact plant (without Agrobacterium); lane WTd: control infiltration of a detached leaf (without Agrobacterium); lane S: purified CMG2-Fc protein standard (2000 ng). Purified CMG2-Fc was used as a positive control to allow comparisons between the relative band intensities. Approximate sizes (kDa) of proteins are shown.