Figure 3. .

Transfection of MCC69B and 624.38-Mel metastatic melanoma cells with microphthalmia-associated transcription factor (MITF) small interfering RNA (siRNA) specifically diminishes progestagen-associated endometrial protein (PAEP) gene expression. (A) Four duplex siRNA; siMITF5, 6, 7, and 8; and a pool of siMITF5-8 were separately transfected into melanoma cells. MITF expression was examined by real-time qRT-PCR at 48 h after transfection. The expression of MITF and PAEP genes was examined by real-time qRT-PCR (B) and Western blotting (C) after siMITF5-8 transfection in melanoma cells at 48 h and 72 h after transfection, respectively. The expression of PAEP and MITF genes was examined by real-time qRT-PCR (D) and Western blotting (E) after siPAEP10-12 transfection in melanoma cells. Mock (wild-type) and nonspecific siControl transfection served as negative controls.