Figure 4. .

Silencing of microphthalmia-associated transcription factor (MITF) or progestagen-associated endometrial protein (PAEP) gene expression decreases the migration of MCC69B and 624.38-Mel metastatic melanoma cells. 624.38-Mel cells with or without transfection of small interfering (si) PAEP10-12 or siControl (A), or with or without transfection of siMITF5-8 or siControl (B), were plated onto the upper transwell chambers and allowed to migrate for 6 h. 624.38-Mel (C) or MCC69B (D) cells that migrated to the underside of the chamber were counted in 10 randomly chosen fields under a microscope and were analyzed by Dunnett t-test, with the relative migration ratio of wild-type cells set as 100% (P < .05).