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. Author manuscript; available in PMC: 2012 Sep 20.
Published in final edited form as: Neurosci Lett. 2011 Aug 4;502(3):192–196. doi: 10.1016/j.neulet.2011.07.043

Figure 1. AAV-mediated depletion of BDNF in the VTA and timeline of experiments.

Figure 1

A - Percent control BDNF expression (pg BDNF/mg protein). Infusion of AAV-GFP-shRNA into the VTA reduced BDNF by approximately 36% (*p < 0.05; n = 6–10) as measured by BDNF ELISA in animals euthanized 3 weeks after viral infusion. B - BDNF immunolabeling in the intact VTA and C - near the injection site of AAV-GFP-shRNA 3 weeks earlier (contralateral side of the same section as shown in B); magnification 200X. D - Fluorescence image of GFP expression in rat VTA 3 weeks after viral infusion; magnification 100X. E -Timeline of behavioral experiments.