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. Author manuscript; available in PMC: 2012 Sep 20.
Published in final edited form as: Biochemistry. 2011 Aug 23;50(37):8005–8017. doi: 10.1021/bi201043j

Figure 2.

Figure 2

Representative analytical HPLC traces (220 nm absorption) of His6HD5, purified HD5red and purified HD5ox (variable sample concentrations). TCEP was added to Ni-NTA purified His6-HD5 to provide the fully reduced sample His6-HD5red (75 mM HEPES buffer, pH 7.4).