Figure 1. Representative ‘Add-back’ experiment.

CD34⁺, CD3⁺, CD14⁺, CD56⁺ and CD19⁺ cells were isolated from CB MNC by MACS. CD34⁺ cells were then cultured alone or in the presence of CD3⁺ ± CD14⁺ ± CD56⁺ ± CD19⁺ cells at levels comparable to those present in the initial CB MNC. While CD34⁺ ex vivo expansion was reduced in the presence of CD3⁺ or CD14⁺ cells, maximal reduction was observed when CD3⁺ and CD14⁺ cells were present (indicated by arrows).