CD34+ cell numbers are shown for each condition following expansion (mean±SEM, n=4-6 replicate experiments. *Statistically-significant from MNC, P≤0.05). Fold increase from input (dashed line) to output is also shown. CD34+ cell expansion was approximately 2.5, 2.3 and 2.5-fold greater than MNC following CD3, CD14, and CD3 & CD14 depletion, respectively.