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Col1a1 expression increased in loaded ulnae at 1, 6, 8, 12, and 32 days. qPCR was used to evaluate Col1a1 gene expression in loaded and control ulnae across the time course. Col1a1 expression was normalized to β-actin expression to facilitate comparison among samples. A paired t test was used to compare expression in loaded and control conditions (ap < .05). Standard errors are indicated.
