Table 1.
The 11 Enzymes and Dpp Used to Digest the FRET Peptides
| Enzyme | Dspp-FRET | MutDspp-FRET |
|---|---|---|
| MMP-2 | MQGDDPN | - |
| MMP-8 | MQGDDPN | - |
| MMP-9 | MQGDDPN | - |
| Mmp-20 | - | - |
| MT1-MMP | - | - |
| MT3-MMP | - | - |
| Klk-4 | SMQGDDPN | SIEGDDPN |
| Plasmin | SMQGDDPN | SIEGDDPN |
| BMP1 | DDPN | - |
| MEP1A | DDPN | - |
| MEP1B | DDPN | DDPN |
The amino acid sequences of the C-terminal cleavage products of Dspp-FRET (Abz-YEFDGKSMQGDDPN-KDnp) and mutDspp-FRET (Abz-YEFDGKSIEGDDPN-KDnp) were obtained by collecting the UV peaks not associated with a fluorescent peak and characterizing them by Edman degradation. A dash indicates that only the uncleaved peptide was identified at the end of the incubation.