Fig. 6.

Increased osteoclastogenesis in EP1^−/− mice is not a cell-autonomous process. Splenocytes were isolated from 2-month-old EP1^−/− mice and control C57BL/6J mice. Cells were cultured at 50,000 cells/well in α-MEM containing 10% FBS and 10 ng/mL of M-CSF in 96-well plates. Then 50 ng/mL of RANKL was added to induce osteoclastogenesis. Five days after culture, TRAcP staining was performed (A, B). Representative photographs are shown at 100× original magnification. Similar numbers of osteoclasts were observed in EP1^−/− and wild-type cultures (C). The data in panel C represent the mean of four different experiments (8 culture wells per group per experiment).