Fig. 4.

MEK inhibitor diminishes the inhibitory effect of SDC-1 siRNA on apoptosis of MCF-7 cells. Cells were pretreated with PD98059 (50 μM) for 24 h and then transfected with SDC-1 siRNA for 48 h. (A) Caspases-3/7 activity was determined with Caspase-Glo® 3/7 assay following the manufacture’s protocol. Data are means ± standard errors. of three experiments performed in triplicate. Values with different letters are significantly different (P < 0.05). (B) Apoptotic activity was determined by cleaved PARP using western blots. Data are presented relative to control (=1.0) and are means ± standard errors of two independent experiments.