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. 2011 Aug 16;8:6. doi: 10.1186/1742-4933-8-6

Figure 2.

Figure 2

IFNg secretion by antigen-specific immunodominant and subdominant CD8+ T cells. CD8+ T cells were stimulated weekly for 3 weeks with M158-66-aAPC (left panels), with pool 1 aAPC, which included NA231-239-aAPC, PA225-233-aAPC, and PB1413-421-aAPC (middle panels), or with pool 2 aAPC, which included NA75-84-aAPC, PA46-54-aAPC, and NS1123-132-aAPC (right panels). A) IFNg production by aAPC stimulated CD8+ T cells from donor C1 and donor C7 after three rounds of stimulation as determined by ICS. CD8+ T cells were stimulated with unpulsed T2 cells, M158-66 pulsed T2 cells, PB1413-421 pulsed T2 cells, or PA46-54 pulsed T2 cells at a 1:1 ratio. B and C) Naïve CD8+ T cells were separated and then stimulated weekly for three weeks with pool 1 aAPC. B) IFNg production by aAPC stimulated memory CD8+ T cells from donor C1. CD8+ T cells were stimulated with unpulsed T2 cells or PB1413-421 pulsed T2 cells. C) IFNg production by aAPC stimulated naïve CD8+ T cells from donor C1. Naïve selected CD8+ T cells were stimulated with unpulsed or PB1413-421 pulsed T2 cells.