Table 1.
Change in milk protein expression on 2 and 14 days post-intramammary infection with different S.aureus strains
| Spot I.D. | Trmt*Day | LSM ± SEM | p-vlaue | Direction |
|---|---|---|---|---|
| 11 | P*14 | 0.03 ± 0.01 | 0.0205 | ↑ |
| Heba*14 | 0.02 ± 0.01 | 0.0382 | ↑ | |
| 12 | P*2 | 0.05 ± 0.02 | 0.0127 | ↑ |
| 13 | P*2 | 0.02 ± 0.01 | 0.0206 | ↑ |
| Heba*14 | 0.02 ± 0.01 | 0.024 | ↑ | |
| 21 | P*14 | 0.03 ± 0.01 | 0.0397 | ↑ |
| 33 | P*2 | 0.05 ± 0.02 | 0.0056 | ↑ |
| 35 | NB*2 | 0.02 ± 0.004 | 0.0041 | ↑ |
| Heba*14 | 0.01 ± 0.005 | 0.0467 | ↑ | |
| 39 (CPP3) | P*2 | 0.02 ± 0.007 | 0.0476 | ↑ |
| P*14 | 0.01 ± 0.004 | 0.0032 | ↑ | |
| Heba*14 | 0.01 ± 0.004 | 0.0154 | ↑ | |
| 45 | NB*2 | 0.01 ± 0.004 | 0.0122 | ↑ |
| 131 | NB*2 | 0.01 ± 0.005 | 0.031 | ↑ |
| 141 | NB*2 | 0.04 ± 0.01 | 0.0296 | ↑ |
| 212 | P*2 | 0.01 ± 0.004 | 0.0038 | ↑ |
| Heba*14 | 0.01 ± 0.005 | 0.0408 | ↑ | |
| 220 | P*2 | 0.04 ± 0.01 | 0.0214 | ↑ |
| Heba*14 | 0.01 ± 0.005 | 0.0304 | ↑ | |
| 222 | NB*2 | 0.02 ± 0.009 | 0.0384 | ↑ |
| 227 | P*2 | 0.008 ± 0.003 | 0.0127 | ↑ |
| 235 (CPP3) | P*2 | 0.03 ± 0.01 | 0.0241 | ↑ |
| P*14 | 0.01 ± 0.006 | 0.0492 | ↑ | |
| Heba*14 | 0.02 ± 0.006 | 0.0127 | ↑ | |
| 244 | P*14 | -0.008 ± 0.002 | 0.0016 | ↓ |
| 250 | NB*2 | 0.007 ± 0.003 | 0.0417 | ↑ |
| NB*14 | 0.01 ± 0.005 | 0.0535 | ↑ | |
| 251 | P*2 | 0.04 ± 0.02 | 0.0449 | ↑ |
| (β-casein precursor) | P*14 | 0.008 ± 0.002 | 0.0025 | ↑ |
| 341 | P*14 | 0.02 ± 0.004 | 0.0006 | ↑ |
| 343 | P*14 | 0.02 ± 0.009 | 0.0188 | ↑ |
| 346 | NB*2 | 0.02 ± 0.01 | 0.0331 | ↑ |
| 349 (CPP3) | NB*2 | 0.01 ± 0.004 | 0.0084 | ↑ |
| 350 | NB*2 | 0.02 ± 0.01 | 0.0423 | ↑ |
| 351 | P*2 | 0.04 ± 0.01 | 0.0079 | ↑ |
| P*14 | 0.02 ± 0.01 | 0.0467 | ↑ | |
| 352 | P*2 | 0.01 ± 0.006 | 0.0411 | ↑ |
| 354 | NB*2 | -0.02 ± 0.004 | < 0.0001 | ↓ |
| (β-Lactoglobulin) | NB*14 | -0.02 ± 0.007 | 0.0105 | ↓ |
| Heba*14 | 0.01 ± 0.007 | 0.0459 | ↑ | |
| 355 | NB*2 | 0.01 ± 0.005 | 0.0481 | ↑ |
| 358 | NB*2 | 0.05 ± 0.02 | 0.0216 | ↑ |
| 359 | NB*2 | 0.01 ± 0.005 | 0.0494 | ↑ |
| P*2 | 0.01 ± 0.005 | 0.0474 | ↑ | |
NB, Newbould 305; P, Parent 3231; Heba, SCV Heba 3231
Trmt*Day denotes the treatment and day of the observed change in protein spot
CPP3, component 3 of the proteose peptone
Data are presented as the least square mean ± standard error of the mean (LSM ± SEM).
Direction indicates the up-or down-regulation of the protein spot on day 2 or 14 pi, compared to baseline.
Spot I.D. number refers to protein spot number assigned by the Melanie software. Spots that were excised and identified by LC-MS/MS are indicated in parentheses.