Table 1. General sequences of microarray supported oligonucleotides and primer sequences.
1-member oligonucleotide microarray |
5′-TCCCAGGGAAAGCATGGAAGAAGGAGAACCTTCTCTCTCTCTCTCTCTCT-3′ |
10-member oligonucleotide microarray |
5′-TCCCAGGGAAAGCATGGHHHHHHHHHHHHCTTCTCTCTCTCTCTCTCTCT-3′ |
3,875 and 10,000-member oligonucleotide microarrays |
5′ AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCTGG- |
HHHHHHHHHHHH CTTAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG-3′ |
Primer-1 |
5′-TCCCAGGGAAAGCATGG-3′ |
Primer-2 |
5′-AGAGAGAGAGAGAGAGAGAAG-3′ |
Primer-2-FAM |
5′-FAM-AGAGAGAGAGAGAGAGAGAAG-3′ |
Solexa-primer-1 |
5′-AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT-3′ |
Solexa-primer-2 |
5′-CAAGCAGAAGACGGCATACGAGCTCTTCCGATCT-3′ |
Primer-3 |
5′-CTACACGACGCTCTTCCGATCTGG-3′ |
Primer-4-FAM |
5′-FAM-GCATACGAGCTCTTCCGATCTAAG-3′ |
H = A, C, or T.