Table 1. Pairwise bacterial coaggregation.
S. oralis | S. sanguinis | S. mitis | S. downei | |||||||||
t1 | t2 | t3 | t1 | t2 | t3 | t1 | t2 | t3 | t1 | t2 | t3 | |
A. naeslundii | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 3 | 3 | 0 | 0 | 0 |
S. downei | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | - | - | - |
S. mitis | 0 | 0 | 0 | 0 | 0 | 0 | - | - | - | - | - | - |
S. sanguinis | 0 | 0 | 0 | - | - | - | - | - | - | - | - | - |
Pairwise coaggregation of A. naeslundii AK6, S. downei HG 594, S. mitis SK24, S. sanguinis SK150 and S. oralis SK248. Coaggregation was determined 15 min, 2 h and 24 h after mixing in 1/10 diluted Todd Hewitt Broth. t1 = 15 min; t2 = 2 h; t3 = 24 h. Grade 0: No visible aggregates in cell suspension. Grade 1: Small uniform aggregates in suspension. Grade 2: Definite coaggregates easily seen, but suspension remained turbid without immediate settling of coaggregates. Grade 3: Large coaggregates which settled rapidly leaving some turbidity in the supernatant fluid. Grade 4: Clear supernatant fluid and large coaggregates which settled immediately.