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. 2011 Sep 23;6(9):e25299. doi: 10.1371/journal.pone.0025299

Table 1. Pairwise bacterial coaggregation.

S. oralis S. sanguinis S. mitis S. downei
t1 t2 t3 t1 t2 t3 t1 t2 t3 t1 t2 t3
A. naeslundii 0 0 0 0 1 1 2 3 3 0 0 0
S. downei 0 0 0 0 0 0 0 0 0 - - -
S. mitis 0 0 0 0 0 0 - - - - - -
S. sanguinis 0 0 0 - - - - - - - - -

Pairwise coaggregation of A. naeslundii AK6, S. downei HG 594, S. mitis SK24, S. sanguinis SK150 and S. oralis SK248. Coaggregation was determined 15 min, 2 h and 24 h after mixing in 1/10 diluted Todd Hewitt Broth. t1 = 15 min; t2 = 2 h; t3 = 24 h. Grade 0: No visible aggregates in cell suspension. Grade 1: Small uniform aggregates in suspension. Grade 2: Definite coaggregates easily seen, but suspension remained turbid without immediate settling of coaggregates. Grade 3: Large coaggregates which settled rapidly leaving some turbidity in the supernatant fluid. Grade 4: Clear supernatant fluid and large coaggregates which settled immediately.