Figure 6. Asymmetric division of GFP+ cells to produce a GFAP+/nestin+ radial type cell and a neuronal cell.

A: Time-lapse imaging of GFP+ cell division in a hippocampal slice from a P5 nestin-GFP Tg mouse (see Video S4). B: 20 hours after cell division, the slice was fixed and then processed for immunohistochemistry. Optical images (C, D): The cells in B indicated by arrowheads C and D are located at different levels of the Z-axis. Each cell is shown separately in different single optical images of C and D. The GFP-positive cells (arrowheads C and D) in B correspond to those indicated by arrowheads in C and D, respectively. One GFP+ daughter cell had a radial process and an astrocytic cell marker (GFAP+) and a progenitor cell marker (nestin+). Another GFP+ daughter cell expressed the neuronal marker Hu+, but was negative for GFAP and nestin, suggesting neuronal differentiation. Scale bar, 10 µm.