Table 1. Antibodies.
Marker | Species, isotype | Label | Working dilution | Vendor |
Primary antibodies | ||||
GFAP | Guinea pig IgG | None | 1∶800 | Advanced ImmunoChem., CA, USA |
GFP | Rat IgG | None | 1∶400 | Nakalai Tesque, Japan |
Hu | Human IgG | None | 1∶2000 | Gift from Dr. H. J. Okano |
Ki67 | Mouse IgG | None | 1∶100 | Novocastra, UK |
Nestin | Mouse IgG | None | 1∶2000 | BD Bioscience, CA, USA |
NeuroD | Goat IgG | None | 1∶400 | Santa Cruz Biotech., CA, USA |
Sox2 | Rabbit IgG | None | 1∶1000 | Chemicon, CA, USA |
Tbr2 | Rabbit IgG | None | 1∶2000 | Gift from Dr. R. Hevner |
Secondary antibodies | ||||
Anti-goat IgG | Donkey IgG | Cy5 | 1∶200 | Jackson, PA, USA |
Anti-guinea pig IgG | Donkey IgG | Cy3 | 1∶200 | Jackson |
Anti-guinea pig IgG | Donkey IgG | Biotin | 1∶200 | Jackson |
Anti-human IgG | Donkey IgG | Cy5 | 1∶200 | Jackson |
Anti-mouse IgG | Donkey IgG | Cy5 | 1∶200 | Jackson |
Anti-mouse IgG | Donkey IgG | Biotin | 1∶200 | Jackson |
Anti-rabbit IgG | Donkey IgG | Cy3 | 1∶200 | Jackson |
Anti-rat IgG | Donkey IgG | Cy2 | 1∶200 | Jackson |