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. 2011 Sep 23;6(9):e25303. doi: 10.1371/journal.pone.0025303

Table 1. Antibodies.

Marker Species, isotype Label Working dilution Vendor
Primary antibodies
GFAP Guinea pig IgG None 1∶800 Advanced ImmunoChem., CA, USA
GFP Rat IgG None 1∶400 Nakalai Tesque, Japan
Hu Human IgG None 1∶2000 Gift from Dr. H. J. Okano
Ki67 Mouse IgG None 1∶100 Novocastra, UK
Nestin Mouse IgG None 1∶2000 BD Bioscience, CA, USA
NeuroD Goat IgG None 1∶400 Santa Cruz Biotech., CA, USA
Sox2 Rabbit IgG None 1∶1000 Chemicon, CA, USA
Tbr2 Rabbit IgG None 1∶2000 Gift from Dr. R. Hevner
Secondary antibodies
Anti-goat IgG Donkey IgG Cy5 1∶200 Jackson, PA, USA
Anti-guinea pig IgG Donkey IgG Cy3 1∶200 Jackson
Anti-guinea pig IgG Donkey IgG Biotin 1∶200 Jackson
Anti-human IgG Donkey IgG Cy5 1∶200 Jackson
Anti-mouse IgG Donkey IgG Cy5 1∶200 Jackson
Anti-mouse IgG Donkey IgG Biotin 1∶200 Jackson
Anti-rabbit IgG Donkey IgG Cy3 1∶200 Jackson
Anti-rat IgG Donkey IgG Cy2 1∶200 Jackson