Skip to main content
. 2011 Sep 23;6(9):e25412. doi: 10.1371/journal.pone.0025412

Figure 3. Lack of p53 expression in mast cells results in enhanced kinase activity of IKK2 and degranulation.

Figure 3

(A, B) IgE-mediated SNAP-23 phosphorylation in WT and p53−/− BMMCs was analyzed by immunoblotting using phosphorylation site-specific antibody. The representative blots from 4 independent experiments (A) and means ± SD of the density of the blots (B) are shown. *significantly different from the mean value of WT BMMCs, *p<0.01. d.u. = density unit. (C) WT and p53−/− BMMCs were incubated with ML120B (10 µM) or vehicle (DMSO (0.01%)). Sixty minutes later, IgE-mediated degranulation was assessed as described in Figure 2D . Data are means ± SD of the percent β-hexosaminidase release, n = 5, *p<0.05. n.s. = not significant.