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. Author manuscript; available in PMC: 2013 Feb 1.
Published in final edited form as: Prostate. 2011 Jun 16;72(3):291–300. doi: 10.1002/pros.21431

Figure 1.

Figure 1

Identification of differentially expressed proteins in the bone marrow samples from men with or without bone metastasis from prostate cancer. A. Elution profiles of the pooled bone marrow supernatants with WGA-agarose affinity chromatography. Bone marrow supernatants from prostate cancer patients who did not have clinical evidence of bone metastasis served as control (Ctrl) for those with bone metastasis (Met). B. SDS-PAGE of the WGA-affinity purified fractions. Arrow indicates a decrease in the level of a 16 kDa protein in the Met samples. Mass spectrometry analysis identified it as hemoglobin β-chain. C. Two-dimensional gel electrophoresis of the proteins eluted from WGA-affinity chromatography. Two discernible protein spots in the Met samples are circled. One of them was identified to be katanin p60 by mass spectrometry analysis.