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. Author manuscript; available in PMC: 2013 Feb 1.
Published in final edited form as: Prostate. 2011 Jun 16;72(3):291–300. doi: 10.1002/pros.21431

Figure 6.

Figure 6

Effect of katanin p60 overexpression on PC-3 and PNT1a cell growth and migration. A. Overexpression of V5-tagged katanin p60 in PC-3 cells by transient transfection of a pcDNA3.1D-KATNA1-V5-6xHis (KATNA1-V5). Recombinant protein and endogenous katanin p60 were detected by immunoblotting with anti-katanin p60 antibody. B. The recombinant protein was confirmed by anti-V5 epitope antibody. C. Overexpression of katanin p60 inhibits cell proliferation in PC-3 cells. Growth curves were recorded from the PC-3 cells transfected with an empty vector or a KATNA1-V5 expression construct. D. Cell migration properties of the transfected PC-3 cells were determined in transwell assays. Three independent experiments for each cell line were performed and a representative experiment with PC-3 cells is shown. *, P < 0.05. E. Recombinant and endogenous katanin p60 in PNT1a cells were detected by immunoblotting with anti-katanin p60 antibody. F. Overexpression of katanin p60 inhibited cell proliferation in PNT1a cells. G. Elevated katanin p60 levels induced an increase in cell migration in PNT1a cells. *, P < 0.05.