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. 2010 Oct 27;41(1):214–223. doi: 10.1002/eji.201040612

Figure 4.

Figure 4

MASP-2A binding to immobilized ficolin-B and ficolin-B mutants using surface plasmon resonance. Equivalent amounts of ficolin-B and ficolin-B mutants (5000–6000 RU) were immobilized on to a CM5 sensor chip and MASP-2A was injected at 0.04, 0.08, 0.16 and 0.33 μM. The dotted line shows the fit to a two-binding site model for ficolin-B. In ficolin-B PO, Lys54 and Ala55 have been mutated to proline and hydroxyproline respectively. In ficolin-B E, Ala55 was replaced by a glutamic acid residue.