FIG. 2.

Histology of bone regenerates with involvement of purified pericytes and Nell-1. (A–F) Only small amounts of cartilaginous tissue (arrows) formed in hCBC alone and hCBC + rhNell-1 implants without loaded pericytes at 2 weeks postoperation. (G–L) Large amount of cartilaginous tissue and focal mineralized woven bone (arrows) formed in pericyte-loaded hCBC implants with even more mineralized bone with Nell-1 presence. (M–R) At 4 weeks postoperation, new bone formation with bone sialoprotein positive staining (arrows) occurred at almost every pericyte-loaded hCBC scaffold implant with the higher rhNell-1dose. Scale bars: 100 μm for A, D, G, and J; 50 μm for B, C, E, F, H, I, K, L, N, O, Q, and R; and 250 μm for M and P. Color images available online at www.liebertonline.com/tea