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. 2011 Jul 14;17(19-20):2497–2509. doi: 10.1089/ten.tea.2010.0705

FIG. 5.

FIG. 5.

Tissue engraftment of implanted human pericytes into regenerates. (A) Images of implants without loaded human pericytes onto hCBC showed cell nucleus stained with Hoechst 33342 (in blue). (B) The RKH live cell dye prelabeled human pericytes (in red) were clearly visible in the implants of pericyte-loaded hCBC groups at 4 weeks postintramuscular implantation. (C) Higher magnification of image B. (D) Prelabeled human pericytes (in red) in hCBC + rhNell-1 implants. (E) Phase-contrast image D showing hCBC particles outlined in yellow lines and tissue profile. (F) Superimposed image of D and E showing implanted pericytes engrafted into remodeling hCBC scaffolds (yellow arrows). (G–I) Prelabeled human pericytes not only engrafted into remodeling hCBC scaffolds (pink arrows), but also participated in vascularization (yellow arrows). (J–L) Prelabeled pericytes combined with CD31 immunohistochemistry (in green), showing colocalization (yellow arrows). Scale bar: 50 μm for A and B, and 25 μm for C–I.