Protein conc.	Ab. dil. IHC	% gel	Ab. dil. Western	Source	Manufacturer
Collagen type I (15 μg)	1:200	4–20	1:1,000	mouse	Sigma, St. Louis. MO
Collagen type IV	1:400	N/A	N/A	goat	Southern Biotech, Birmingham, AL
Collagen type IV(7 μg)	N/A	4–20	1:1,000	mouse	Chemicon, Temecula, CA
Laminin (30 μg)	1:400	7.5	1:1,000	rabbit	Research Diagnostics, Concord, MA
Fibronectin (30 μg)	1:400	7.5	1:1,000	mouse	Research Diagnostics
MMP-2 (50 μg)	1:400	12.5	1:1,000	Mouse	Oncogene, Cambridge, MA
MMP-9 (50 μg)	1:400	12.5	1:1,000	mouse	Oncogene
TIMP-1 (50 μg)	1:100	18	1:100	Mouse	Calbiochem, San Diego, CA
TIMP-2 (50 μg)	1:100	18	1:100	Mouse	Calbiochem
PAI-1 (38 μg)	1:100	15	1:400	goat	Santa Cruz, Santa Cruz, CA
TGF-β (30 μg)	1:400	18	1:500	rabbit	Santa Cruz
TGFβRI (30 μg)	1:200	12.5	1:500	rabbit	Santa Cruz
TGFβRII (30 μg)	1:200	12.5	1:500	rabbit	Santa Cruz
Smad2/3 (30 μg)	1:200	12.5	1:500	goat	Santa Cruz
pSmad2/3 (30 μg)	1:200	12.5	1:500	goat	Santa Cruz
Smad4 (30 μg)	1:200	12.5	1:800	goat	Santa Cruz
Smad6 (30 μg)	1:200	12.5	1:800	goat	Santa Cruz
Smad7 (30 μg)	1:200	12.5	1:800	goat	Santa Cruz
Smurf2 (30 μg)	1:200	12.5	1:1,000	goat	Santa Cruz
CD68	1:400	N/A	N/A	mouse	Serotec, Oxford, UK