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. Author manuscript; available in PMC: 2011 Sep 24.
Published in final edited form as: J Biol Chem. 2005 May 27;280(29):26904–26912. doi: 10.1074/jbc.M413845200

Fig 6. Promoter activity within the 5′ flanking and intron 1 region of the HYAL-2 gene.

Fig 6

C-28/I2 cells were transfected with 0.2 µg of one of the pGL-3 Basic promoter constructs (shown in Fig. 5) or the empty pGL-3 Basic vector alone. Forty-eight hours post-transfection the cells were lysed and assayed for luciferase activity. The values represent the mean ± S.E. of a representative experiment performed in duplicate and are expressed as fold stimulation with respect to pGL3-Basic. The values were normalized to Renilla luciferase activity that was transfected concurrently (2 ng) in all the assays to correct for transfection efficiency. At least 3 sets of independent experiments were performed for each set of constructs. Panel A includes the C-series; panel B, the P-series and; panel C, the L-series. (+) denotes sequence cloned in the forward orientation, (−) denotes sequences cloned in the reverse orientation.