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. Author manuscript; available in PMC: 2011 Sep 24.
Published in final edited form as: J Biol Chem. 2005 May 27;280(29):26904–26912. doi: 10.1074/jbc.M413845200

Fig 8. Effects of IL-1β concentration and incubation time on HYAL-2 mRNA expression.

Fig 8

Human articular chondrocytes (Panel A) and C-28/I2 immortalized human chondrocytes (Panel B) were pre-incubated overnight in serum-free medium followed by subsequent 48 h treatment in the absence or presence of 0.5, 1.0, 5.0 or 10.0 ng/ml IL-1β. In the experiments depicted panel C, following an overnight preincubation in serum-free medium, human articular chondrocytes were treated in the absence or presence of 10.0 ng/ml IL-1β for 3, 6, 13 or 24 h. Total RNA was isolated and RT-PCR was performed to assess the level of HYAL-2 mRNA (upper set of bands) and GAPDH mRNA (lower set of bands) expression. The products were visualized following agarose gel electrophoresis and staining with ethidium bromide.