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. Author manuscript; available in PMC: 2012 Sep 30.
Published in final edited form as: J Neurosci Methods. 2011 Jul 23;201(1):67–77. doi: 10.1016/j.jneumeth.2011.07.012

Figure 2.

Figure 2

Explanting MEAs produces LSCM histology data with variable morphological distortion (a, b), including formerly interfacing tissue now collected in the hole (*) or removed and remaining on the explanted device (c). Fluorescence intensity of an applied marker can be affected by distance from a surface contacting the labeling solution, as shown in (d) where oligodendrocyte cell bodies (arrows) and myelinated branches are more intensely labeled given closer proximity to a large blood vessel (centered) within a 100 μm thick tissue slice. These confounding factors associated with explanting can limit the detailed study of microdevice integration in the CNS. Images in (a, b, d) are single optical sections; image in (c) is a 90 μm highest-intensity z-projection. Microdevices in (a) and (c) were implanted 2 weeks, while device in (b) was implanted 4 weeks. Scale bars are 50 μm (a, c, d), 100 μm (b). (For interpretation of the references to color in this gure legend, the reader is referred to the web version of the article.)