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. 2011 Jul 12;123(2):411–420. doi: 10.1093/toxsci/kfr188

FIG. 3.

FIG. 3.

miR-190 targets PHLPP. (A) Schematic diagram of the miR-190-binding site at the 3′-UTR of PHLPP mRNA. Lower panel shows alignment of the predicted miR-190-binding sites to 3-UTR of PHLPP from different species (Homo sapiens, Macaca mutatta, Rattus norvegicus, Mus musculus, Eguus caballus, and Gallus gallus). (B) Western blotting shows concentration-dependent reduction of the PHLPP protein in the cells treated with As3+. (C) Overexpression of miR-190 downregulates PHLPP. The BEAS-2B cells were mock transfected, or transfected with a miR-190, miR-190In, or treated with 20μM of As3+. The protein level of PHLPP was determined by Western blotting. (D) Overexpression of miR-190, but not miR-190In, downregulates the activity of the PHLPP 3′-UTR reporter gene. (E) Overexpression of miR-190 has no effect on the PHLPP 3′-UTR reporter without the miR-190-binding site.