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. 2011 Aug 26;9:143. doi: 10.1186/1479-5876-9-143

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Copyright ©2011 Chakera et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Effects of storage on assay sensitivity. Whole blood (Panels A & B) or PBMCs (Panels C & D) were stored for variable periods of time at room temperature (RT), 4°C (whole blood) or -80°C (PBMCs) before stimulation with BZLF1 or PHA for 18 hours followed by RNA extraction. Induction of MIG (Panels A&C) and IP-10 (Panels B&D) mRNA transcripts are shown normalized to the negative control (ACTs). Columns show the mean RQ value and 95% confidence intervals calculated from triplicate wells. Results are representative of three individual experiments.