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. 2011 Aug 31;6:10. doi: 10.1186/1750-2187-6-10

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Copyright ©2011 Aksaas et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Localization of GPKOW. Immunofluorescence analysis of paraformaldehyde fixated 293T (rows 1, 4 and 6) and U2OS cells (rows 2, 3 and 5) stained with anti-GPKOW 1894 (rows 1-5, green), anti-V5 (row 6, red) or anti-lamin B (row 6, green). In the fourth row the antibody was preincubated with antigen (+ blocking peptide) before incubation. The three lower rows show anti-GPKOW and anti-V5 staining of transfected full-length (rows 4-5) or V5-tagged (row 6) GPKOW. Nuclear DNA was visualized with Hoechst (rows 1-6, blue). Merged photos to the right. Scale bar, 10 μM.