Table 1.
Serological and genomic tests results of 32 patients reactive on the pepEIA HIV-2 screening assay
| Result of pepEIA screeninga | Result of confirmatory assay | Result of nested PCR (Subtyping) |
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| INNO-LIA HIV 1/2b | ||||||||||||
|
Patient code |
HIV-1 | HIV-2 | HIV type | |||||||||
| HIV-1 OD | HIV-2 OD | HIV-2 OD (1/10)d | p24 | gp41 | gp120 | gp36 | gp105 |
New LAV Blotc II (HIV-2) |
HIV-1 | HIV-2 | ||
| FEH0251 | 2578 | 563 | 155 | 3 | 3 | 3 | neg | neg | 1 | nd | POS (C) | Neg |
| FEH0049 | 2886 | 592 | 356 | 3 | 3 | 3 | neg | neg | 1 | nd | POS (C) | Neg |
| FEH0220 | 2815 | 624 | 196 | 3 | 3 | 3 | neg | neg | 1 | nd | POS (C) | Neg |
| FEH0105 | 2820 | 694 | 283 | 3 | 3 | 3 | neg | neg | 1 | nd | POS (C) | Neg |
| FEH0345 | 2804 | 713 | 230 | 3 | 3 | 3 | neg | neg | 1 | nd | POS (C) | Neg |
| FEH0047 | 3024 | 718 | 310 | 3 | 3 | 3 | 2 | neg | 1-2 | nd | POS (C) | Neg |
| FEH0190 | 2972 | 937 | 144 | 3 | 3 | 3 | 1 | neg | 1-2 | nd | POS (C) | Neg |
| FEH0027 | 2979 | 947 | 273 | 2 | 3 | 3 | neg | neg | 1 | nd | POS (C) | Neg |
| FEH0596 | 2892 | 962 | 160 | 3 | 3 | 3 | neg | 1 | 1-2 | Indeterm | POS (C) | Neg |
| FEH0281 | 2539 | 1086 | 296 | 3 | 3 | 3 | 2 | neg | 1-2 | POS | POS (C) | Neg |
| FEH0226 | 3008 | 1275 | 416 | 3 | 3 | 2 | 2 | neg | 1-2 | POS | POS (C) | Neg |
| FEH0210 | 2820 | 1619 | 393 | 3 | 3 | 3 | 1 | neg | 1-2 | POS | POS (C) | Neg |
| FEH0244 | 2805 | 1695 | 291 | 2 | 3 | +/- | 2 | neg | 1-2 | Indeterm | POS (C) | Neg |
| FEH1044 | 2645 | 1764 | 446 | 3 | 3 | 3 | neg | neg | 1 | nd | POS (C) | Neg |
| FEH0327 | 2885 | 2156 | 389 | 3 | 3 | 2 | neg | neg | 1 | nd | POS (C) | Neg |
| FEH0700 | 2405 | 699 | 116 | +/- | 4 | 2 | neg | 2 | 1-2 | nd | POS (C) | Neg |
| FEH0687 | 2799 | 1485 | 279 | 1 | 4 | 3 | neg | neg | 1 | nd | POS (C) | Neg |
| FEH0086 | 2959 | 1426 | 755 | 3 | 3 | 3 | neg | neg | 1 | nd | POS (C) | Neg |
| FEH0394 | 2794 | 1794 | 864 | 3 | 3 | 3 | 1 | neg | 1-2 | POS | POS (C) | Neg |
| FEH1049 | 3010 | 2146 | 729 | 3 | 3 | 3 | neg | neg | 1 | nd | POS (C) | Neg |
| FEH0875 | 2859 | 2222 | 702 | 2 | 3 | 3 | neg | neg | 1 | nd | POS (C) | Neg |
| FEH0982 | 2412 | 2261 | 1425 | 3 | 3 | 3 | neg | neg | 1 | nd | POS (C) | Neg |
| FEH0404 | 3031 | 2262 | 1054 | 3 | 3 | 3 | neg | neg | 1 | POS | POS (C) | Neg |
| FEH0857 | 2837 | 2272 | 1481 | 3 | 3 | 2 | neg | neg | 1 | nd | POS (C) | Neg |
| FEH0646 | 3008 | 2364 | 573 | 3 | 3 | 3 | neg | neg | 1 | POS | POS (C) | Neg |
| FEH1196 | 3017 | 2620 | 1145 | 2 | 3 | 3 | neg | neg | 1 | Indeterm | POS (C) | Neg |
| FEH1097 | 2996 | 2662 | 547 | 3 | 3 | 3 | neg | neg | 1 | nd | POS (C) | Neg |
| FEH1040 | 2996 | 2697 | 672 | 3 | 3 | 3 | neg | neg | 1 | nd | POS (C) | Neg |
| FEH0681 | 2882 | 2069 | 2988 | 2 | 4 | 3 | 1 | neg | 1-2 | nd | POS (C) | Neg |
| FEH0089 | 2858 | 2809 | 1600 | 3 | 3 | 3 | 3 | 3 | 1-2 | POS | POS (C) | POS (A) |
| FEH0726 | 197 | 2051 | 922 | neg | neg | neg | 3 | 1 | 2 | POS | Neg | POS (A) |
| FEH0924 | 194 | 2779 | 2080 | +/- | neg | neg | 2 | 3 | 2 | POS | Neg | POS (A) |
aUnderlined numbers denotes reactivity on the pepEIA;
bOnly reactivity to antigens of interest is reported;
c nd, not tested; indeterm, indeterminate;
d assay performed with sample further diluted 1/10