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IFN-γ ELISPOT. The statistical results from the ELISPOT of immunized Balb/c mice splenic lymphocyte after stimulation with peptides by Analysis of Variance (ANOVA). The negative control represents lymphocytes plated with RPMI 1640 without peptides. The experimental groups are lymphocytes plated with peptides. Asterisks indicate statistical significance compared with the value of wells without peptides (P<0.01)
