Table 1.

Pten expression status and gene copy number quantitation of Pten and p53 in mutant mouse prostate samples.

Mouse ID (age)	Sample #	Pathology	Pten IHC	Pten Copy #	Normalized Qty (Ren)	p53 Copy #	Normalized Qty (p53)
c-MYC+;Pten-het;p53-ko
7352 (16wks)	S55	PIN	-	0	0.1156	0*	ND
	S56	PIN	-	0	0.2928	0*	ND
	S58	Cancer	-	0	0.0446	0*	ND
7345 (17wks)	S52	PIN	-	0	0.1534	0*	ND
	S53	PIN	-	0	0.2352	0*	ND
	S54	Cancer	-	0	0.007	0*	ND
151 (19wks)	S51	PIN	+	1	0.6712	0*	ND
	S49	PIN	-	0	0.1732	0*	ND
	S50	Cancer	-	0	0.1482	0*	ND
c-MYC+;Pten-ko;p53-het
547 (16wks)	S70	PIN	ND	0*	ND	0	0.2315
	S69	Cancer	ND	0*	ND	0	0.098
593 (18wks)	S100	PIN	ND	0*	ND	1	0.3003
	S101	PIN	ND	0*	ND	1	0.4117
	S102	PIN	ND	0*	ND	1	0.2942
c-MYC+;Pten-het;p53-het
7312 (10wks)	S19	PIN	-	0	0.0015	0	0.0448
8150 (16wks)	S1	Cancer	-	0	0.3333	1	0.5797
	S2	PIN	-	1	0.6062	1	0.6318
	S3	PIN	-	1	0.6087	1	0.7103
	S4	Cancer	-	0	0.3283	1	0.5631
	S5	PIN	+	1	0.9152	1	0.7202
	S6	PIN	+	1	1.0263	1	0.5593
	S7	PIN	+	1	0.9161	1	0.6605
	S8	PIN	+	1	1.0027	1	0.5276
	S10	PIN	+	1	1.3528	1	0.9262
561 (17wks)	S96	PIN	-	1	0.483	1	0.4282
	S97	PIN	-	1	0.5407	1	0.4322
	S98	PIN	-	0	0.3992	1	0.3146
	S99	PIN	-	0	0.4241	1	0.3983
355 (18wks)	S83	PIN	-	0	0.3536	1	0.3051
	S84	PIN	-	1	1.6025	1	0.3974
243 (23wks)	S82	PIN	-	0	0.3355	0	0.236
8184 (24wks)	S11	PIN	-	0	0.2316	1	0.751
	S12	PIN	-	1	0.9762	1	0.6265
	S13	PIN	-	1	0.8407	1	0.6842
	S14	PIN	-	1	0.7727	1	0.7652
	S15	PIN	-	1	0.7679	1	0.701
	S16	PIN	-	1	0.6586	1	0.7117
	S17	PIN	-	1	0.5308	1	0.7464
	S78	PIN	-	0	0.2545	0	0.157
	S79	PIN	-	0	0.2557	1	0.3114
	S80	PIN	-	0	0.2463	1	0.3972
	S81	Cancer	-	0	0.3272	0	0.2112
7367 (26wks)	S28	PIN	+	1	0.4922	1	0.32
	S29	PIN	-	1	0.5657	1	0.483
	S30	PIN	-	0	0.3419	0	0.1239
	S31	PIN	-	0	0.3549	0	0.2659
	S32	PIN	-	0	0.2626	1	0.3308
	S34	PIN	-	1	0.552	1	0.3014
	S35	PIN	+	1	0.7529	0	0.2411
	S36	PIN	-	0	0.4428	1	0.3726
	S38	PIN	+	1	0.5998	1	0.3149
	S39	PIN	-	0	0.4191	1	0.2928
	S41	PIN	+	1	0.4396	1	0.4553
	S42	PIN	-	0	0.3313	1	0.6036
	S76	PIN	-	1	0.3464	0	0.2705
	S77	PIN	-	0	0.3299	0	0.2451
411 (27wks)	S93	PIN	-	1	0.4076	1	0.3423
	S94	PIN	-	0	0.4472	1	0.3562
	S95	PIN	-	0	0.4099	1	0.3082
7379 (38wks)	S43	Cancer	-	0	0.0376	0	0.0159
	S44	Cancer	-	0	0.0168	0	0.0078
	S45	Cancer	-	0	0.0815	0	0.018
	S47	PIN	-	0	0.2102	0	0.1327
8409 (39wks)	S22	Cancer	-	0	0.0001	0	0.1290
	S23	Cancer	-	0	0.0009	0	0.0690
	S24	Cancer	-	0	0.0003	0	0.0691
	S25	Cancer	-	0	0.1867	0	0.2615
109 (40wks)	S86	PIN	-	0	0.2649	0	0.2491
	S87	PIN	-	0	0.0777	0	0.0779
	S88	Cancer	-	0	0.0054	0	0.0076
	S89	Cancer	-	0	0.0348	0	0.0223
	S90	PIN	-	0	0.1058	0	0.0563
	S91	PIN	-	0	0.0825	0	0.0771
	S92	PIN	-	0	0.1826	0	0.1242

Gene copy number was determined by qPCR of DNA isolated from immunofluorescence-guided microdissected PIN and cancer lesions.

^*Copy number based on genotype. ND = not determined. Wks = weeks. IHC=immunohistochemistry.