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. Author manuscript; available in PMC: 2011 Sep 25.
Published in final edited form as: Hum Mutat. 2008 Jun;29(6):861–868. doi: 10.1002/humu.20740

FIGURE 1.

FIGURE 1

Monocytes and T cells in PBMCs have impaired production of cytokines in response to different stimuli. A total of 2 × 106 patient and control PBMCs were stimulated with LPS (1 μg/ml) +IFNγ (1,000 U/ml), SAC (Pansorbin cells, 1:10,000 w/v)+IFNγ, OspA (1 μg/ml) +IFNγ, or CD40L (3.5 μg/ml)+IFNγ. TNFα and IL-12 production were measured at 36 hr by ELISA. TNFα and IL-12 production from unstimulated (control) conditions were <50 pg/ml and <10 pg/ml, respectively, for both patient and control. Data are representative of two independent experiments. b: Patient and control T cells in 2 × 106 PBMCs were stimulated with plate coated OKT3 or OKT3+IL-12 (10 μg/ml). Production of IFNγ and TNFα was analyzed by ELISA after 36 hr. IFNγ and TNFα production from unstimulated conditions was<50 pg/ml for both patient and control. Data are representative of two independent experiments.