Skip to main content
. Author manuscript; available in PMC: 2011 Sep 25.
Published in final edited form as: Hum Mutat. 2008 Jun;29(6):861–868. doi: 10.1002/humu.20740

FIGURE 3.

FIGURE 3

c.40G>T IKBA mutation results in an N-terminal truncated IκBα that exerts a dominant negative effect on NF-κB signaling. a: Cotransfection experiment in HEK293 cells with pNF-kB-Luc reporter plasmid and either pCMV-IKBA-WT or pCMV-IKBA-40G>T. Luciferase activity was measured in response to TNFα stimulation. The luciferase activity of each sample was normalized to an internal reference standard of β-galactosidase activity. Data represent one of three independent cotransfection experiments. b: c.40G>T IKBA encodes a truncated IκBα protein .293 cells were transfected with C-terminal V5-His tagged expression vectors with the wild-type IKBA or the c.40G>T IKBA mutant allele. Protein expression was analyzed by immunoprecipitation with an anti-His antibody in cellular lysates followed by immunoblotting with a C-terminal IKBA antibody.