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. Author manuscript; available in PMC: 2012 Sep 15.
Published in final edited form as: Anal Chem. 2011 Aug 17;83(18):7086–7093. doi: 10.1021/ac201307d

Figure 3.

Figure 3

a,b) Experimental results for multiplexed concentration enhanced aptamer affinity probe electrophoresis assay for detecting IgE in buffer solution after 30 minutes of concentration, c) Electropherogram for optimized electrokinetic concentration and separation of IgE aptamer (5nM) and different concentrations of IgE: (1) 4.92pM, (2) 0.6 nM, and (3) 6.75 nM IgE. The complex band is labeled with an asterisk, d) Inset demonstrates detection of 4.92 pM IgE in buffer, e) Dose-response curve of anti-IgE aptamer with IgE spiked in buffer, error bars represent standard error from duplicate experiments, f) Linear relationship in the log-log plot is obtained at low concentrations of IgE. The measured LOD is 4.4 pM IgE.