. Author manuscript; available in PMC: 2012 Sep 21.

Published in final edited form as: J Am Chem Soc. 2011 Jul 27;133(37):14476–14479. doi: 10.1021/ja203172c

Table 1.

Comparison of catalytic constants k_cat and K_M determined from the MesaPlate and reported in the literature.¹⁵

Isoform	Substrate	MesaPlate		Literature
Isoform	Substrate	k_cat (min⁻¹)	K_M (μM)	k_cat (min⁻¹)	K_M (μM)
CYP3A4	Testosterone^a	118 ± 8	31 ± 7	105	24
CYP3A4	Dextromethorphan^a	38 ± 8	106 ± 64	27	210
CYP2C9	Diclofenac	11 ± 1	20 ± 7	29	5
CYP2C9	Tolbutamide	6 ± 1	366 ± 154	10	103

Literature values for overall substrate depletion were calculated from published data on multiple product formation as described in Supporting Information.