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. Author manuscript; available in PMC: 2011 Dec 30.
Published in final edited form as: Nature. 2011 Jun 29;474(7353):658–661. doi: 10.1038/nature10195

Figure 3. Vpx programs SAMHD1 for proteasomal degradation in MDM, via CRL4DCAF1 E3.

Figure 3

a. SAMHD1 levels in MDM infected with 3-fold serial dilutions of SIV VLP loaded with wild type or Q76A substituted HIV-2 Rod Vpx, or in mock infected MDM (M). α-tubulin served as loading control.

b. SAMHD1 levels in MDM infected with 3-fold dilutions of SIVmac Vpx-loaded SIV VLP and cultured in the presence or absence of MG132 proteasome inhibitor (1 μg/ml).

c. Vpx depletes SAMHD1 levels in MDM via DCAF1. MDM were subjected to RNAi targeting DCAF1, SAMHD1 or non-targeting RNAi (NT1, NT2), and infected (+) or not (-) with SIV VLP loaded with SIVmac Vpx, two days later. DCAF1 and SAMHD1 were revealed by Western blotting after additional two days.