Skip to main content
. Author manuscript; available in PMC: 2012 Oct 1.
Published in final edited form as: Neurobiol Dis. 2011 Jun 25;44(1):73–83. doi: 10.1016/j.nbd.2011.06.006

Figure 4. AAV-Netrin-1 gene transfer leads to an increase in transduced Netrin-1 protein.

Figure 4

A, AAV-Netrin-1 gene therapy resulted in a significant increase in transduced Netrin-1 protein (detected by goat-anti-chicken netrin-1) in the peri-infarct cortex of dMCAO rats by western blotting 3 weeks following AAV-LacZ or AAV-Netrin-1 injection. (n=3 per group). B, Confocal images showing double immunofluorescence staining of chicken netrin-1 (green) with cell type specific markers (red) including NeuN, GFAP, Oligo, Oligo2, NG2 or RECA. The results suggest that the transduced netrin-1 following AAV gene transfer was predominantly expressed by neurons. (n=3 per group). Scale bars: 10 μm.