Figure 3.
Effect of α-tocopherol, CO2-SFE mussel oil, and 5β-scymnol on TNF-α release in UVB-irradiated (a) HEM and (b) MM96L cells. Cell cultures were incubated with 0.625 μg/mL of test compounds for 24 h prior to and after UVB-irradiation (2 kJ/m2) with or without 10 ng/mL IL-1α. After incubation, the media samples were collected and microconcentrated for ELISA, and the cell lysates were used to determine total protein concentration. Results were expressed as TNF-α released (pg)/cell protein (mg) and represent means ± SD of triplicate samples. Statistical analysis was performed using Student's paired t-test where significance was recorded as P ≤ 0.05. Significant difference between UVB-irradiated untreated sample and test compound-treated irradiated sample in the (∗) absence of IL-1α or (†) presence of IL-1α.