Skip to main content
. Author manuscript; available in PMC: 2012 Feb 5.
Published in final edited form as: Cell. 2011 Jul 21;146(3):448–461. doi: 10.1016/j.cell.2011.06.041

Figure 5. TRAF2 and TRAF6 are recruited to MAVS aggregates in response to virus infection.

Figure 5

(A) Crude mitochondria (P5) were isolated from cells infected with Sendai virus or not infected, solubilized in 1% DDM and then separated by sucrose gradient ultracentrifugation. Fractions were analyzed by immunoblotting with the indicated antibodies. Aliquots of the fractions were incubated with cytosolic extracts, 35S-IRF3 and ATP followed by native gel electrophoresis. (B) Similar to (A), except that cells were transfected with siRNA against MAVS or GFP (control) before virus infection.