Figure 3. Glial glutamate transporters regulate the magnitude of tonic IGLU.
Aa, bath application of the glial-selective glutamate transporter blocker dihydrokainate (DHK, 300 μm) induced an inward shift in Iholding. Ab, group data showing a significant DHK-mediated increase in Iholding (n = 10). Ac, example showing a DHK-mediated membrane depolarization and firing discharge in an SON neuron. Ba, bath application of the selective gliotoxin α-aminoadipic acid (αAA, 2 mm) induced a similar inward shift in Iholding as that evoked by DHK. Bb, group data showing a significant αAA-mediated increase in Iholding (n = 7). Bc, example showing an αAA-mediated membrane depolarization and firing discharge in an SON neuron. Note that the DHK and αAA effects were blocked by KYN. *P < 0.001 and **P < 0.0001 vs. basal, paired t test. All recordings shown obtained at a holding Vm of −70 mV.