Figure 4.

Western blot and mRNA expression analysis for FADD in MMC. A. The cytosolic and membrane bound lyastes after transfection of MMC with siRNA-EphA2 or siRNA-Control or MMC alone were subjected to Western analysis. CMT is Camptothecin (10µM/L) was used as a positive control. β-actin served as a loading control. B. FADD mRNA expression in MMC was determined by quantitative real time PCR. The data presented are mean ± SEM of three independent experiments. ** P < 0.05 compared with MMC only; * P < 0.05 compared to siRNA-EphA2 versus siRNA-EphA2 + z-IETD-fmk; Cyt-Fr = cytoplasmic fraction; Mem-Fr = membrane fraction; z-IETD-fmk = caspase-8 inhibitor.